منابع مشابه
Enzyme Localization during Melanogenesis
The DOPA-reaction was used to identify tyrosinase in the nucleus and cytoplasm of the neural crest melanoblast of Taricha torosa, the California newt. In this urodele there is a nuclear DOPA-positive response during the normal embryonic development from the late blastula stage to the nucleus of the early melanocyte. During the gastrula stages, all nuclei of this newt are DOPA-positive. This pos...
متن کاملEnzyme localization in Bacillus megaterium.
Weibull (1953a) has shown that after osmotic lysis of protoplasts of Bacillus megaterium, prepared by controlled treatment with lysozyme, only two microscopically detectable structures remain; membrane-like bodies termed "ghosts," and granules. The ghosts were found to sediment as a dark yellow fraction by centrifugation at 10,000 G (Weibull, 1953b) and to contain almost all the pigmented mater...
متن کاملMorphological localization of the bacteriophage tail enzyme.
In an earlier report dealing with the chemistry of viral invasion, Barrington and Kozloff (1,2) showed that incubat,ion of various T series bacteriophages with N16-labeled Escherichiu coli B cell walls caused the release of as much as 15 per cent of the total nitrogen from the host cell walls. The characteristics of this interaction appeared to be similar to those of an enzymatic reaction, and ...
متن کاملStudy of lipoprotein lipase content in Ob17 preadipocytes during adipose conversion. Immunofluorescent localization of the enzyme.
The development of lipoprotein lipase has been examined during adipose conversion of preadipocyte Ob17 cells. These cells have been previously shown to differentiate in clusters of fat cells. The lipoprotein lipase activity, which increases 20-50-fold during adipose conversion, is fully inhibited by anti-lipoprotein lipase y-globulins (directed against the rat heart enzyme) and is heparin-relea...
متن کاملEnhanced ultrastructural localization of acid phosphatase using microwave irradiation during enzyme incubation
Microwave irradiation in enzyme cytochemistry is not only advantageous for shortening procedures, but will also improve the sensitivity of histochemical detection since short periods and less fixative are required to obtain good preservation. Moreover, shorter incubation procedures will reduce the diffusion and loss of enzyme activity. Here we compared conventional incubations in a shaking wate...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1969
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.43.2.220